Rhodopsin-cyclases for photocontrol of cGMP/cAMP and 2.3 A structure of the adenylyl cyclase domain.
Scheib U, Broser M, Constantin OM, Yang S, Gao S, Mukherjee S, Stehfest K, Nagel G, Gee CE, Hegemann P
Nat Commun. 2018 May 24;9(1):2046. doi: 10.1038/s41467-018-04428-w.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 101720 | pAAV hSyn1 CaRhGC wt T2A tDimer | humanized, Neuron-specific promoter driven expression of the rhodopsin guanylyl cyclase from Catenaria anguillulae with a neuron-specific promoter. Useful for raising intracellular cAMP close to the m |
| 101721 | pAAV hSyn1 YFP-CaRhAC | Yellow fluorescent protein (YFP) – tagged rhodopsin adenylyl cyclase created from the rhodopsin guanylyl cyclase of Catenaria anguillulae with a neuron-specific promoter. Useful for raising intracellu |
| 101722 | pAAV hSyn1 CaRhAC T2A tDimer | - humanized - variant of pAAV hSyn YFP-CaRhAC Addgene # 101721– less reliable in hippocampal neurons |