Applying the auxin-inducible degradation system for rapid protein depletion in mammalian cells.

Lambrus BG, Moyer TC, Holland AJ
Methods Cell Biol. 2018;144:107-135. doi: 10.1016/bs.mcb.2018.03.004. Epub 2018 Apr 5. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
80073	pBabe Blast osTIR1-9Myc	Retroviral vector for stable expression of osTIR1-9Myc in mammalian cell lines
80074	pBabe Puro osTIR1-9Myc	Retroviral vector for stable expression of osTIR1-9Myc in mammalian cell lines
101713	pcDNA5/FRT miniAID-EGFP	For amplification of a miniAID-EGFP for C-terminal tagging
101714	pcDNA5/FRT EGFP-miniAID	For amplification of a EGFP-miniAID for N-terminal tagging
101715	px459 VQR	sgRNA/Cas9 expression plasmid with Cas9 VQR mutations (NGA PAM)
101716	px459 VRER	sgRNA/SpCas9 expression plasmid with Cas9 VRER mutations (NGCG PAM)
101727	p458 VQR	Expresses a sgRNA and a Cas9 VQR variant that recognizes "NGA" PAM motifs
101728	p458 VRER	Expresses a sgRNA and a Cas9 VRER variant that recognizes "NGCG" PAM motifs
101729	px330 VRER	Expresses a sgRNA and a Cas9 VRER variant that recognizes "NGCG" PAM motifs
101730	p330 VQR	Expresses a sgRNA and a Cas9 VQR variant that recognizes "NGA" PAM motifs
101731	px458 EQR	Expresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifs
101732	px459 EQR	Expresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifs
101733	px330 EQR	Expresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifs