Adaptation of the GoldenBraid modular cloning system and creation of a toolkit for the expression of heterologous proteins in yeast mitochondria.
Perez-Gonzalez A, Kniewel R, Veldhuizen M, Verma HK, Navarro-Rodriguez M, Rubio LM, Caro E
BMC Biotechnol. 2017 Nov 13;17(1):80. doi: 10.1186/s12896-017-0393-y.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 101682 | pUPD-pGAL1 (01-11) | pGAL1 in domestication vector |
| 101683 | pUPD-pTDH3 (01-11) | pTDH3 in domestication vector |
| 101684 | pUPD-pTDH2 (01-11) | pTDH2 in domestication vector |
| 101685 | pUPD-pTEF2 (01-11) | pTEF2 in domestication vector |
| 101686 | pUPD-pHXT7 (01-11) | pHXT7 in domestication vector |
| 101687 | pUPD-pTPI1 (01-11) | pTPI1 in domestication vector |
| 101688 | pUPD-pPYK1 (01-11) | pPYK1 in domestication vector |
| 101689 | pUPD-PGK1 (01-11) | pPGK1 in domestication vector |
| 101690 | pUPD-PGI1 (01-11) | pPGI1 in domestication vector |
| 101691 | pUPD-Su9MTS (12) | Su9MTS in domestication vector |
| 101692 | pUPD-SOD2MTS (12) | SOD2MTS in domestication vector |
| 101693 | pUPD-MAM33MTS (12) | MAM33MTS in domestication vector |
| 101694 | pUPD2-ODPAMTS (12) | ODPAMTS in domestication vector |
| 101695 | pUPD2-ODPBMTS (12) | ODPBMTS in domestication vector |
| 101696 | pUPD2-ATPAMTS (12) | ATPAMTS in domestication vector |
| 101697 | pUPD2-GLRX2MTS (12) | GLRX2MTS in domestication vector |
| 101698 | pUPD-MTS2 (12) | MTS2 in domestication vector |
| 101699 | pUC57-8xHis | 8xHis in domestication vector |
| 101700 | pUPD-Cyc1t (17-21) | Cyc1t in domestication vector |
| 101701 | pUPD-ADH2t (17-21) | ADH2t in domestication vector |
| 101702 | pUPD-TDH2t (17-21) | TDH2t in domestication vector |
| 101703 | pUPD-G418R (01-21) | G418R in domestication vector |
| 101704 | pUPD-HygroBR (01-21) | HygroBR in domestication vector |
| 101705 | YORWΔ22Ω2 | GoldenBraid vector pDGB2Ω2, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YORWΔ22 solo LTR loci |
| 101706 | YORWΔ22Ω1 | GoldenBraid vector pDGB2Ω1, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YORWΔ22 solo LTR loci |
| 101707 | YORWΔ22α2 | GoldenBraid vector pDGB2α2, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YORWΔ22 solo LTR loci |
| 101708 | YORWΔ22α1 | GoldenBraid vector pDGB2α1, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YORWΔ22 solo LTR loci |
| 101709 | YPRCΔ15Ω2 | GoldenBraid vector pDGB2Ω2, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YPRCΔ15 solo LTR loci |
| 101710 | YPRCΔ15Ω1 | GoldenBraid vector pDGB2Ω1, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YPRCΔ15 solo LTR loci |
| 101711 | YPRCΔ15α2 | GoldenBraid vector pDGB2α2, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YPRCΔ15 solo LTR loci |
| 101712 | YPRCΔ15α1 | GoldenBraid vector pDGB2α1, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YPRCΔ15 solo LTR loci |