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Dynamic Blue Light-Inducible T7 RNA Polymerases (Opto-T7RNAPs) for Precise Spatiotemporal Gene Expression Control.

Baumschlager A, Aoki SK, Khammash M
ACS Synth Biol. 2017 Nov 17;6(11):2157-2167. doi: 10.1021/acssynbio.7b00169. Epub 2017 Oct 18. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
101661pAB150Opto-T7RNAP*(563)
101662pAB147Opto-T7RNAP*(302)
101663pAB152Opto-T7RNAP*(563-F2)
101664pAB153Opto-T7RNAP*(600)
101665pAB144Opto-T7RNAP*(69)
101666pAB151Opto-T7RNAP*(563-F1)
101667pAB164Opto-T7RNAP*(563), half expr.
101668pAB166Opto-T7RNAP*(563-F2), half expr.
101669pAB170Opto-T7RNAP*(69), half expr.
101670pAB171Opto-T7RNAP*(69), equal expr.
101671pAB168Opto-T7RNAP*(563-F1), equal expr.
101672pAB167Opto-T7RNAP*(563), equal expr.
101673pAB174Opto-T7RNAP*(179)
101674pAB202Opto-T7RNAP(563)
101675pAB203Opto-T7RNAP(563-F1)
101676pSKA322empty backbone with chloramphenicol resistance
101677pAB213mCherry under T7 promoter control
101678pAB50mCherry under T7 promoter control
101679pAB143LAA-tagged mCherry under T7 promoter control

Antibodies from Article