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Vectors for Expression of Signal Peptide-Dependent Proteins in Baculovirus/Insect Cell Systems and Their Application to Expression and Purification of the High-Affinity Immunoglobulin Gamma Fc Receptor I in Complex with Its Gamma Chain.

Le LTM, Nyengaard JR, Golas MM, Sander B
Mol Biotechnol. 2018 Jan;60(1):31-40. doi: 10.1007/s12033-017-0041-8. (Link opens in a new window) PubMed

Plasmids from Article

ID Plasmid Purpose
101651pGS_N_StrepExpression of type I signal-peptide containing proteins with N-terminal Twin-Strep-tag in insect cells
101652pGS_C_StrepExpression of type I signal-peptide containing proteins with C-terminal Twin-Strep-tag in insect cells
101653pGS_C_3xFLAGExpression of type I signal-peptide containing proteins with C-terminal 3xFLAG-tag in insect cells
101654pGS_N_3xFLAGExpression of type I signal-peptide containing proteins with N-terminal 3xFLAG-tag in insect cells
109191pGS-CD64-2Encodes full-length engineered CD64 with C-terminal Twin-Strep tag to be expressed in a baculovirus/insect cell expression system
109192pGS-FCER1G-1Encodes human full-length FCER1G to be expressed in a baculovirus/insect cell expression system

Antibodies from Article