Rapid and scalable preparation of bacterial lysates for cell-free gene expression.
Didovyk A, Tonooka T, Tsimring L, Hasty J
ACS Synth Biol. 2017 Aug 10. doi: 10.1021/acssynbio.7b00253.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 99244 | pAD-LyseR | For autolysate production in E. coli BL21-Gold (DE3) strain: constitutively expresses phage lambda endolysin (gene R) |
| 99245 | pAD-LyseR (in lacZ deficient strain) | For autolysate production in lacZ deficient (lac operon deletion) E. coli BL21-Gold-dLac (DE3) strain: constitutively expresses phage lambda endolysin (gene R) |
| 99246 | pAD-GamS | Expresses 6xHis-tagged GamS from T7lac promoter in the E.coli Tuner (DE3) strain. See notes below for important strain information. |
| 99247 | LacZ deficient (lac operon deletion) BL21-Gold-dLac (DE3) strain | LacZ deficient (lac operon deletion) BL21-Gold-dLac (DE3) strain |