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Fast two-photon imaging of subcellular voltage dynamics in neuronal tissue with genetically encoded indicators.

Chamberland S, Yang HH, Pan MM, Evans SW, Guan S, Chavarha M, Yang Y, Salesse C, Wu H, Wu JC, Clandinin TR, Toth K, Lin MZ, St-Pierre F
eLife. 2017 Jul 27;6. pii: e25690. doi: 10.7554/eLife.25690. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
101274pcDNA3.1/Puro-CAG-ASAP2sGenetically encoded voltage indicator ASAP2s expressed under strong mammalian promoter (CAG)
101275pAAV-CaMKII-ASAP2sGenetically encoded voltage indicator (GEVI) ASAP2s in AAV vector for expression in excitatory glutamatergic neurons using the promoter CaMKII
101276pAAV-hSyn-ASAP2sGenetically encoded voltage indicator (GEVI) ASAP2s in AAV vector for neuron-specific expression using the promoter hSyn1

Antibodies from Article