Molecular mechanism of extreme mechanostability in a pathogen adhesin.
Milles LF, Schulten K, Gaub HE, Bernardi RC
Science. 2018 Mar 30;359(6383):1527-1533. doi: 10.1126/science.aar2094.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 101238 | pET28a-SdrG_N2N3-HIS-ybbr | SdrG N2 and N3 domains with C-terminal ybbr tag for covalent immobilization |
| 101239 | pET28a-Fgß-ddFLN4-HIS-ybbr | wildtype Fgß peptide with ddFLN4 fingerprint domain with C-terminal ybbr tag for covalent immobilization - resulting in high forces |
| 101240 | pET28a-FFSARG-ddFLN4-HIS-ybbr | minimal SdrG binding peptide FFSARG with ddFLN4 fingerprint domain with C-terminal ybbr tag for covalent immobilization - resulting in high forces |
| 101717 | pET28a-ClfB_N2N3-HIS-ybbr | ClfB N2 and N3 domains with C-terminal ybbr tag for covalent immobilization |
| 101718 | pET28a-ybbr-HIS-ddFLN4-K10 | K10 peptide with ddFLN4 fingerprint domain and N-terminal ybbr tag for covalent immobilization - resulting in high forces |
| 101719 | pET28a-ybbr-HIS-ddFLN4-Fgß | wildtype Fgß peptide with ddFLN4 fingerprint domain with N-terminal ybbr tag for covalent immobilization - resulting in weak forces |
| 101743 | pET28a-FgßF3-ddFLN4-HIS-ybbr | mutant FgßF3 peptide (additional phenylalanine) with ddFLN4 fingerprint domain with C-terminal ybbr tag for covalent immobilization - resulting in high forces |