A versatile one-step CRISPR-Cas9 based approach to plasmid-curing.
Lauritsen I, Porse A, Sommer MOA, Norholm MHH
Microb Cell Fact. 2017 Aug 2;16(1):135. doi: 10.1186/s12934-017-0748-z.
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PubMed
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 92050 | pFREE | Allows inducible expression of gRNAs and Cas9 for plasmid curing. |
| 92051 | pFREE_Amp | Allows inducible expression of gRNAs and Cas9 for plasmid curing. |
| 92052 | pFREE_Cm | Allows inducible expression of gRNAs and Cas9 for plasmid curing. |
| 92053 | pFREE_Zeo | Allows inducible expression of gRNAs and Cas9 for plasmid curing. |