Improved methods for marking active neuron populations.
Moeyaert B, Holt G, Madangopal R, Perez-Alvarez A, Fearey BC, Trojanowski NF, Ledderose J, Zolnik TA, Das A, Patel D, Brown TA, Sachdev RNS, Eickholt BJ, Larkum ME, Turrigiano GG, Dana H, Gee CE, Oertner TG, Hope BT, Schreiter ER
Nat Commun. 2018 Oct 25;9(1):4440. doi: 10.1038/s41467-018-06935-2.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 101060 | pAAV_hsyn_NES-his-CaMPARI2-WPRE-SV40 | AAV vector expressing CaMPARI2 (Kd = 200nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons |
| 101061 | pAAV_hsyn_NES-his-CaMPARI2-F391W-WPRE-SV40 | AAV vector expressing CaMPARI2_F391W (Kd = 110nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons |
| 101062 | pAAV_hsyn_NES-his-CaMPARI2-H396K-WPRE-SV40 | AAV vector expressing CaMPARI2_H396K (Kd = 360nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons |
| 101063 | pAAV_hsyn_NES-his-CaMPARI2-F391W-G395D-WPRE-SV40 | AAV vector expressing CaMPARI2_F391W-G395D (Kd = 530nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons |
| 101064 | pAAV_hsyn_NES-his-CaMPARI2-L398T-WPRE-SV40 | AAV vector expressing CaMPARI2_L398T (Kd = 825nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons |
| 119723 | pAAV_hsyn_NES-his-CaMPARI2-F391W-L398V | AAV vector expressing CaMPARI2_F391W-L398V (Kd = 174nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons |
| 137185 | pTol2-HuC(elavl3)-CaMPARI2 | pan-neuronal expression of CaMPARI2 in zebrafish, used to generate the CaMPARI2 zebrafish line at ZIRC (ZL13801) |