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Myosin IIA Heavy Chain Phosphorylation Mediates Adhesion Maturation and Protrusion in Three Dimensions.

Rai V, Thomas DG, Beach JR, Egelhoff TT
J Biol Chem. 2017 Feb 24;292(8):3099-3111. doi: 10.1074/jbc.M116.733402. Epub 2017 Jan 4. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
101040pEGFP-NMHC-IIA-S1916AExpresses GFP-MYH9 construct (human myosin IIA) carrying mutation of serine 1916 to alanine, driven by CMV promoter. Thus inhibits PKC phosphorylation of the tail. Also carries S1915A mutation.
101041pEGFP-NMHC-IIA-3xAExpresses GFP-MYH9 construct (human myosin IIA) carrying S1943A mutation and S1916A, S1915A mutation, driven by CMV promoter. Thus inhibits CKII and PKC phosphorylation of the tail.

Antibodies from Article