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Quantitative analysis of the bidirectional viral G-protein-coupled receptor and lytic latency-associated nuclear antigen promoter of Kaposi's sarcoma-associated herpesvirus.

Hilton IB, Dittmer DP
J Virol. 2012 Sep;86(18):9683-95. doi: 10.1128/JVI.00881-12. Epub 2012 Jun 27. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
101197pDD2000Name: pBluescript-LANApi,K14. Bidirectional reporter driving LANApi side [green luciferase isoform from pCBG68] and K14 side [red luciferase isoform from pCBR] in pBluescript backbone.
101198pDD2002Name: pCBG68-LANApi. LANApi driving green luciferase reporter for CBG68 in pCBG68 backbone.
101219pDD2005Name: pCBR-K14. K14 driving red luciferase reporter for CBR in pCBR backbone.
101364pDD2013Name: pCBG68-K14ΔTATA. K14 TATA box mutation in pCBG68-LANApi (pDD2002).
101365pDD2015Name: pCBR-K14ΔTATA. K14 TATA box mutation in pCBR-K14 (pDD2005).
101366pDD2022Name: pCBG68-RBPjkΔB. RBPjk binding site B mutation in pCBG68-LANApi (pDD2002).
101367pDD2023Name: pCBR-RBPjkΔB. RBPjk binding site B mutation in pCBR-K14 (pDD2005).
101368pDD2024Name: pCBG68-RBPjkΔA. RBPjk binding site A mutation in pCBG68-LANApi (pDD2002).
101369pDD2026Name: pCBG68-LANApiΔTATA. LANApi TATA box mutation in pCBG68-LANApi (pDD2002).
101370pDD2027Name: pCBR-LANApiΔTATA. LANApi TATA box mutation in pCBR-K14 (pDD2005).
101371pDD2028Name: pCBR-RBPjkΔA. RBPjk binding site A mutation in pCBR-K14 (pDD2005).
101372pDD2029Name: pBluescript-LANApiΔTATA. LANApi TATA box mutation in pBluescript-LANApi,K14 (pDD2000).
101373pDD2030Name: pBluescript-RBPjkΔB. RBPjk binding site B mutation in pBluescript-LANApi,K14 (pDD2000).
101374pDD2031Name: pBluescript-RBPjkΔA. RBPjk binding site A mutation in pBluescript-LANApi,K14 (pDD2000).
101377pDD2034Name: pBluescript-Δ611-843REV. Blue Heron Biotechnology Inc. bidirectional mutant variant of pBluescript-LANApi,K14 (pDD2000), 611-843 REV.
101378pDD2038Name: pBluescript-ΔRRE. Blue Heron Biotechnology Inc. bidirectional mutant variant of pBluescript-LANApi,K14 (pDD2000), RRE.
101379pDD2045Name: pBluescript-empty vector. pBluescript-LANApi,K14 (pDD2000) bidirectional empty vector for backbone only.

Antibodies from Article