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Generation and characterization of a human iPSC cell line expressing inducible Cas9 in the "safe harbor" AAVS1 locus.

Castano J, Bueno C, Jimenez-Delgado S, Roca-Ho H, Fraga MF, Fernandez AF, Nakanishi M, Torres-Ruiz R, Rodriguez-Perales S, Menendez P
Stem Cell Res. 2017 May;21:137-140. doi: 10.1016/j.scr.2017.04.011. Epub 2017 Apr 22. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
107270pAAVS1-TRE-Cas9- puro-polyA-CAG-rtTAExpresses Cas9 upon induction with doxicycline.

Antibodies from Article