Systematic comparison of 2A peptides for cloning multi-genes in a polycistronic vector.
Liu Z, Chen O, Wall JBJ, Zheng M, Zhou Y, Wang L, Ruth Vaseghi H, Qian L, Liu J
Sci Rep. 2017 May 19;7(1):2193. doi: 10.1038/s41598-017-02460-2.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 111771 | pGEMT-Mef2c-tPT2A-GFP | Bicistronic construct: Co-express two genes by 2A peptides |
| 111772 | pGEMT-3P2A | Quadcistronic construct: Co-express four genes by 3 P2A peptides |
| 111773 | pGEMT-PTE2A | Quadcistronic construct: Co-express four genes by three 2A peptides -- P2A, T2A and E2A |
| 111812 | pGEMT-PT2A-GFP-Tdtomato-iRFP670 | Tricistronic construct: Co-express three fluorescent proteins by P2A and then T2A |
| 111813 | pMXs-PT2A-GFP-iRFP670-Tdtomato | Tricistronic construct: Co-express three fluorescent proteins by P2A and then T2A |
| 111814 | pGEMT-PT2A-Tdtomato-GFP-iRFP670 | Tricistronic construct: Co-express three fluorescent proteins by P2A and then T2A |
| 111815 | pGEMT-PT2A-Tdtomato-iRFP670-GFP | Tricistronic construct: Co-express three fluorescent proteins by P2A and then T2A |
| 111816 | pMXs-PT2A-iRFP670-GFP-Tdtomato | Tricistronic construct: Co-express three fluorescent proteins by P2A and then T2A |
| 111817 | pGEMT-PT2A-iRFP670-Tdtomato-GFP | Tricistronic construct: Co-express three fluorescent proteins by P2A and then T2A |
| 111818 | pGEMT-TPE2A-Mef2c-Tdtomato-Gata4-Tbx5 | Quadcistronic construct: Co-express four genes by three 2A peptides -- T2A, P2A and E2A |