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Widespread Influence of 3'-End Structures on Mammalian mRNA Processing and Stability.

Wu X, Bartel DP
Cell. 2017 May 18;169(5):905-917.e11. doi: 10.1016/j.cell.2017.04.036. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
120162pCMV-GFP-CENPBpAEGFP followed by the poly(A) site from human CENPB gene
120179pCMV-GFP-CENPBpA-fixed-length-clone-1Randomly picked clone 1 of the pCMV-GFP-CENPBpA-fixed-length library
120180pCMV-GFP-CENPBpA-fixed-length-clone-2Randomly picked clone 2 of the pCMV-GFP-CENPBpA-fixed-length library
120181pCMV-GFP-CENPBpA-fixed-length-clone-3Randomly picked clone 3 of the pCMV-GFP-CENPBpA-fixed-length library
120182pCMV-GFP-CENPBpA-fixed-length-clone-4Randomly picked clone 4 of the pCMV-GFP-CENPBpA-fixed-length library
120183pCMV-GFP-CENPBpA-fixed-length-clone-5Randomly picked clone 5 of the pCMV-GFP-CENPBpA-fixed-length library
120184pCMV-GFP-CENPBpA-fixed-length-clone-6Randomly picked clone 6 of the pCMV-GFP-CENPBpA-fixed-length library
120185pCMV-GFP-CENPBpA-fixed-length-clone-7Randomly picked clone 7 of the pCMV-GFP-CENPBpA-fixed-length library
120186pCMV-GFP-CENPBpA-fixed-length-clone-8Randomly picked clone 8 of the pCMV-GFP-CENPBpA-fixed-length library
120187pCMV-GFP-CENPBpA-fixed-length-clone-9Randomly picked clone 9 of the pCMV-GFP-CENPBpA-fixed-length library
120188pCMV-GFP-CENPBpA-fixed-length-clone-10Randomly picked clone 10 of the pCMV-GFP-CENPBpA-fixed-length library
120189pCMV-GFP-CENPBpA-fixed-length-clone-11Randomly picked clone 11 of the pCMV-GFP-CENPBpA-fixed-length library
120190pCMV-GFP-CENPBpA-fixed-length-clone-12Randomly picked clone 12 of the pCMV-GFP-CENPBpA-fixed-length library
120191pCMV-GFP-CENPBpA-fixed-length-clone-13Randomly picked clone 13 of the pCMV-GFP-CENPBpA-fixed-length library
120192pCMV-GFP-CENPBpA-fixed-length-clone-14Randomly picked clone 14 of the pCMV-GFP-CENPBpA-fixed-length library
120193pCMV-GFP-CENPBpA-delhuman CENPB poly(A) site reporter, with stem loop deleted
120194pCMV-GFP-RPS5pAHuman RPS5 poly(A) site reporter
120196pCMV-GFP-RPS5pA-m1Human RPS5 poly(A) site reporter, stem loop mutant 1
120197pCMV-GFP-RPS5pA-m1rHuman RPS5 poly(A) site reporter, stem loop mutant 1 rescue
120198pCMV-GFP-RPS5pA-m2Human RPS5 poly(A) site reporter, stem loop mutant 2
120199pCMV-GFP-RPS5pA-m2rHuman RPS5 poly(A) site reporter, stem loop mutant 2 rescue
120200pCMV-GFP-RPS5pA-delhuman RPS5 poly(A) site reporter, with stem loop deleted
120201pCMV-GFP-TFE3pAHuman TFE3 poly(A) site reporter
120202pCMV-GFP-TFE3pA-G9CHuman TFE3 poly(A) site reporter, stem loop mutant
120203pCMV-GFP-TFE3pA-G9C-C19GHuman TFE3 poly(A) site reporter, stem loop mutant rescue
120204pCMV-GFP-TFE3pA-delhuman TFE3 poly(A) site reporter, with stem loop deleted
120205pCMV-GFP-ILVBLpAHuman ILVBL poly(A) site reporter
120206pCMV-GFP-ILVBLpA-G10CHuman ILVBL poly(A) site reporter, stem loop mutant
120207pCMV-GFP-ILVBLpA-G10C-C21GHuman ILVBL poly(A) site reporter, stem loop mutant rescue
120208pLentiCRISPRv1-CENPBgRNA targeting the stem loop of CENPB poly(A) site
120209CRISPRi-CENPB-g1CENPB CRISPRi guide RNA 1
120210CRISPRi-CENPB-g2CENPB CRISPRi guide RNA 2
120211CRISPRi-CENPB-g3CENPB CRISPRi guide RNA 3
120212CRISPRi-CENPB-g4CENPB CRISPRi guide RNA 4
120213CRISPRi-CENPB-g5CENPB CRISPRi guide RNA 5
120214CRISPRi-CENPB-g6CENPB CRISPRi guide RNA 6
120215CRISPRi-CENPB-g7CENPB CRISPRi guide RNA 7
120216CRISPRi-CENPB-g8CENPB CRISPRi guide RNA 8
120217CRISPRi-CENPB-g9CENPB CRISPRi guide RNA 9
120218CRISPRi-CENPB-g10CENPB CRISPRi guide RNA 10

Antibodies from Article