Synthetic mRNA devices that detect endogenous proteins and distinguish mammalian cells.
Kawasaki S, Fujita Y, Nagaike T, Tomita K, Saito H
Nucleic Acids Res. 2017 May 19. doi: 10.1093/nar/gkx298.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
140266 | plet7d_stbC-EGFP | LIN28-dependent translational repression |
140267 | pLin28A-myc-T2A-tagRFP | Expresses LIN28A in mammalian cells |
140268 | pU1utr-EGFP | U1A-dependent translational repression |
140269 | pU1A-myc-T2A-tagRFP | Expresses truncated U1A in mammalian cells |
140278 | pTAPmyc-T2A-tagRFP | Backbone for making a mammalian expression vector of the protein of interest |
140288 | pAptamerCassette-EGFP | Backbone for making protein-responsive mRNA switches |