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Synthetic mRNA devices that detect endogenous proteins and distinguish mammalian cells.

Kawasaki S, Fujita Y, Nagaike T, Tomita K, Saito H
Nucleic Acids Res. 2017 May 19. doi: 10.1093/nar/gkx298. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
140266plet7d_stbC-EGFPLIN28-dependent translational repression
140267pLin28A-myc-T2A-tagRFPExpresses LIN28A in mammalian cells
140268pU1utr-EGFPU1A-dependent translational repression
140269pU1A-myc-T2A-tagRFPExpresses truncated U1A in mammalian cells
140278pTAPmyc-T2A-tagRFPBackbone for making a mammalian expression vector of the protein of interest
140288pAptamerCassette-EGFPBackbone for making protein-responsive mRNA switches

Antibodies from Article