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Enhancement of single guide RNA transcription for efficient CRISPR/Cas-based genomic engineering.

Ui-Tei K, Maruyama S, Nakano Y
Genome. 2017 Jun;60(6):537-545. doi: 10.1139/gen-2016-0127. Epub 2017 Jan 26. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
215847pSuperCRISPR-sgRNA-RL69-WT(TTTT)This plasmid encodes sgRNA that target Renilla luciferase with stretch sequence
215848pSuperCRISPR-sgRNA-RL69-ATTTThis plasmid encodes sgRNA that target Renilla luciferase with stretch sequence
215849pSuperCRISPR-sgRNA-RL69-TATTThis plasmid encodes sgRNA that target Renilla luciferase with stretch sequence
215850pSuperCRISPR-sgRNA-RL69-TTATThis plasmid encodes sgRNA that target Renilla luciferase with stretch sequence
215851pSuperCRISPR-sgRNA-RL69-TTTAThis plasmid encodes sgRNA that target Renilla luciferase with stretch sequence
215852pSuperCRISPR-sgRNA-RL69-CTTTThis plasmid encodes sgRNA that target Renilla luciferase with stretch sequence
215853pSuperCRISPR-sgRNA-RL69-TCTTThis plasmid encodes sgRNA that target Renilla luciferase with stretch sequence
215854pSuperCRISPR-sgRNA-RL69-TTCTThis plasmid encodes sgRNA that target Renilla luciferase with stretch sequence
215855pSuperCRISPR-sgRNA-RL69-TTTCThis plasmid encodes sgRNA that target Renilla luciferase with stretch sequence
215856pSuperCRISPR-sgRNA-EGFP12-WT(TTTT)This plasmid encodes sgRNA that target EGFP with stretch sequence
215857pSuperCRISPR-sgRNA-EGFP12-TTTAThis plasmid encodes sgRNA that target EGFP with stretch sequence
215858pSuperCRISPR-sgRNA-RL864-WT(TTTT)This plasmid encodes sgRNA that target Renilla luciferase with stretch sequence
215859pSuperCRISPR-sgRNA-RL864-TTTAThis plasmid encodes sgRNA that target Renilla luciferase with stretch sequence
215860pSuperCRISPR-sgRNA-RL864R-WT(TTTT)This plasmid encodes sgRNA that target Renilla luciferase with stretch sequence
215861pSuperCRISPR-sgRNA-RL864R-TTTAThis plasmid encodes sgRNA that target Renilla luciferase with stretch sequence
215862pSuperCRISPR-sgRNA-RL554-WT(TTTT)This plasmid encodes sgRNA that target Renilla luciferase with stretch sequence
215863pSuperCRISPR-sgRNA-RL554-TTTAThis plasmid encodes sgRNA that target Renilla luciferase with stretch sequence
215864pSuperCRISPR-sgRNA-RL661-WT(TTTT)This plasmid encodes sgRNA that target Renilla luciferase with stretch sequence
215865pSuperCRISPR-sgRNA-RL661-TTTAThis plasmid encodes sgRNA that target Renilla luciferase with stretch sequence
215866pSuperCRISPR-sgRNA-RL714-WT(TTTT)This plasmid encodes sgRNA that target Renilla luciferase with stretch sequence
215867pSuperCRISPR-sgRNA-RL714-TTTAThis plasmid encodes sgRNA that target Renilla luciferase with stretch sequence

Antibodies from Article