RNA Binding and HEPN-Nuclease Activation Are Decoupled in CRISPR-Cas13a.
Tambe A, East-Seletsky A, Knott GJ, Doudna JA, O'Connell MR
Cell Rep. 2018 Jul 24;24(4):1025-1036. doi: 10.1016/j.celrep.2018.06.105.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 100817 | pDuBir-Lbu-dCas13a-avitag | Dual expression of Lbu-dCas13a with a C-terminal avi-tag and BirA to biotinylate Lbu-dCas13a |