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L-SCRaMbLE as a tool for light-controlled Cre-mediated recombination in yeast.

Hochrein L, Mitchell LA, Schulz K, Messerschmidt K, Mueller-Roeber B
Nat Commun. 2018 May 22;9(1):1931. doi: 10.1038/s41467-017-02208-6. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
100537pLH_Scr15CEN/ARS plasmid with expression cassettes for PhyBNT-CreN and PIF3-NLS-CreC fusion proteins for red/far-red light-regulated Cre recombinase activity in Saccharomyces cerevisiae.
100538pLH_Scr16CEN/ARS plasmid with PhyBNT-NLS-CreN and PIF3-NLS-CreC expression cassettes for red/far-red light-regulated Cre recombinase activity in Saccharomyces cerevisiae.
100539pLM494CEN/ARS plasmid harboring expression cassettes for crtI, crtYB, crtE and tHMG1 of the β-carotene pathway, each flanked by loxPsym sites.

Antibodies from Article