In trans paired nicking triggers seamless genome editing without double-stranded DNA cutting.
Chen X, Janssen JM, Liu J, Maggio I, 't Jong AEJ, Mikkers HMM, Goncalves MAFV
Nat Commun. 2017 Sep 22;8(1):657. doi: 10.1038/s41467-017-00687-1.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 100284 | AL05_pDonor.DMD | DMD intron 43-targeting plasmid |
| 100287 | AL62_pDonor.DMD.TS | DMD intron 43-targeting plasmid |
| 100288 | AC62_pDonor.DMD.TS.DR | DMD intron 43-targeting plasmid |
| 100289 | AX44_pS.Donor.S1 | AAVS1-targeting plasmid |
| 100290 | AX53_pS.Donor.S1.TS | AAVS1-targeting plasmid |
| 100291 | AY42_pS.Donor.R5 | CCR5-targeting plasmid |
| 100292 | AY10_pS.Donor.R5.TS | CCR5-targeting plasmid |
| 100293 | AL08_pgRNA.DMD | DMD intron 43-targeting gRNA expression plasmid |
| 100294 | AY22_pgRNA.R5.1 | CCR5-targeting gRNA expression plasmid |
| 100295 | AY23_pgRNA.R5.2 | CCR5-targeting gRNA expression plasmid |
| 100296 | AV11_pDonor.EP.S1 | AAVS1-targeting expression plasmid |
| 100297 | AV09_pDonor.EP.S1.TS | AAVS1-targeting expression plasmid |