AAV-mediated direct in vivo CRISPR screen identifies functional suppressors in glioblastoma.
Chow RD, Guzman CD, Wang G, Schmidt F, Youngblood MW, Ye L, Errami Y, Dong MB, Martinez MA, Zhang S, Renauer P, Bilguvar K, Gunel M, Sharp PA, Zhang F, Platt RJ, Chen S
Nat Neurosci. 2017 Aug 14. doi: 10.1038/nn.4620.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 100275 | pAAV-U6sgp53-U6sgbbSapI-GFAPCre | AAV-CRISPR mediated genome editing in glia / astrocytes |
| 100276 | pAAV-U6sgp53-mTSG-GFAPCre | AAV-CRISPR library for pool mutagenesis of top tumor suppressor genes |
| 100277 | pLenti-U6sgbbBsmbI-puro-2A-Fluc | Lentiviral vector for CRISPR mediated gene editing in cells while expressing Firefly Luciferase for in vivo imaging |