Life-Long Genetic and Functional Access to Neural Circuits Using Self-Inactivating Rabies Virus.
Ciabatti E, Gonzalez-Rueda A, Mariotti L, Morgese F, Tripodi M
Cell. 2017 Jul 1. pii: S0092-8674(17)30697-9. doi: 10.1016/j.cell.2017.06.014.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
99608 | pSAD-F3-NPEST-iCRE-2A-mCherryPEST | Self-inactivating DG-Rabies (SiR) expressing iCRE and destabilised mCherry |
99609 | pSAD-F3-NPEST-FLPo-2A-mCherryPEST | Self-inactivating DG-Rabies (SiR) expressing FLPo and destabilised mCherry |
99610 | pLenti-CMV-puro-2A-TEVp | Lentivirus expressing puromycin resistance gene and TEV protease |
102984 | pLenti-CMV-GFP-2A-oG-WPRE | Lentivirus expressing eGFP and optimised Rabies Glycoprotein (oG) |
102985 | pAAV-CMV-FLEX-TVAmCherry-2A-oG | AAV expressing TVA-mCherry fusion and optimised Rabies Glycoprotein (oG) after CRE-mediated recombination |
102986 | pAAV-CMV-FRT-myrSNAP-2A-H2BeGFP-WPRE | AAV expressing membrane-targeted SNAP protein and nuclear-targeted eGFP after FLP-mediated recombination |
102987 | pLenti-CMV-CAG-GFP-EnvA | Lentivirus expressing eGFP and (EnvA-B19 Rabies Glycoprotein) fusion protein. Used to package EnvA pseudotyped G-deleted Rabies |
104330 | pAAV-CMV-TVAmCherry-2A-oG | AAV constitutively expressing TVA-mCherry fusion and optimised Rabies Glycoprotein (oG) |
104332 | pSAD-F3-NPEST-mCherry | Self-inactivating DG-Rabies (SiR) expressing mCherry |