Multiplexed in vivo homology-directed repair and tumor barcoding enables parallel quantification of Kras variant oncogenicity.
Winters IP, Chiou SH, Paulk NK, McFarland CD, Lalgudi PV, Ma RK, Lisowski L, Connolly AJ, Petrov DA, Kay MA, Winslow MM
Nat Commun. 2017 Dec 12;8(1):2053. doi: 10.1038/s41467-017-01519-y.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 99848 | AAV-KrasWT/sgKras/Cre | Expresses Cre-recombinase, a barcoded HDR template that serves as a control for HDR into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR. |
| 99849 | AAV-KrasG12A/sgKras/Cre | Expresses Cre-recombinase, a barcoded HDR template to introduce a G12A mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR. |
| 99850 | AAV-KrasG12C/sgKras/Cre | Expresses Cre-recombinase, a barcoded HDR template to introduce a G12C mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR. |
| 99851 | AAV-KrasG12D/sgKras/Cre | Expresses Cre-recombinase, a barcoded HDR template to introduce a G12D mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR. |
| 99852 | AAV-KrasG12R/sgKras/Cre | Expresses Cre-recombinase, a barcoded HDR template to introduce a G12R mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR. |
| 99853 | AAV-KrasG12S/sgKras/Cre | Expresses Cre-recombinase, a barcoded HDR template to introduce a G12S mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR. |
| 99854 | AAV-KrasG12V/sgKras/Cre | Expresses Cre-recombinase, a barcoded HDR template to introduce a G12V mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR. |
| 99855 | AAV-KrasG13A/sgKras/Cre | Expresses Cre-recombinase, a barcoded HDR template to introduce a G13A mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR. |
| 99856 | AAV-KrasG13C/sgKras/Cre | Expresses Cre-recombinase, a barcoded HDR template to introduce a G13C mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR. |
| 99857 | AAV-KrasG13D/sgKras/Cre | Expresses Cre-recombinase, a barcoded HDR template to introduce a G13D mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR. |
| 99858 | AAV-KrasG13R/sgKras/Cre | Expresses Cre-recombinase, a barcoded HDR template to introduce a G13R mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR. |
| 99859 | AAV-KrasG13S/sgKras/Cre | Expresses Cre-recombinase, a barcoded HDR template to introduce a G13S mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR. |
| 99860 | AAV-KrasG13V/sgKras/Cre | Expresses Cre-recombinase, a barcoded HDR template to introduce a G13V mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR. |