Targeted DNA methylation in vivo using an engineered dCas9-MQ1 fusion protein.
Lei Y, Zhang X, Su J, Jeong M, Gundry MC, Huang YH, Zhou Y, Li W, Goodell MA
Nat Commun. 2017 Jul 11;8:16026. doi: 10.1038/ncomms16026.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 89633 | pcDNA3.1-dCas9-MQ1(WT)-EGFP | Targeted CpG methylation |
| 89635 | pcDNA3.1-dCas9-MQ1(C141S)-EGFP | Targeted CpG methylation |
| 89636 | pcDNA3.1-dCas9-MQ1(S317A)-EGFP | Targeted CpG methylation |
| 89637 | pcDNA3.1-dCas9-dMQ1-EGFP | Targeted CpG methylation |
| 89793 | pLV hUbC-dCas9-MQ1(Q147L)-EGFP | Targeted CpG methylation |
| 89794 | pLV hUbC-dCas9-dMQ1-EGFG | Targeted CpG methylation |