George Church Lab: Church Lab CRISPR plasmids 2017
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 99651 | pAAV-dSa-VPR | Expresses tripartite VP64-p65-RTA activator fused to C term. of dead Sa Cas9 |
| 99652 | CRISPR-Sa-TdTom. Reporter | Red fluorescent reporter that can be activated by dSa Cas9 activator indicating activity |
| 99696 | pAAV-SCP1-dSa VPR mini.-2X snRP-1 Afp | Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Afp, vector allows for strong activation of mouse Afp, can be packaged and delivered as AAV |
| 99697 | pAAV-CMV-dSa VP64 Afp | Expresses dSa Cas9 fused to gold standard VP64 activator domain and Sa sgRNA for Afp promoter, vector allows for activation of mouse Afp, can be packaged and delivered as AAV |
| 99698 | pAAV-SCP1-dSa VPR mini.-2X snRP-1 BsaI gRNA | Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA with BsaI cloning sites for programming, vector allows for strong activation of programmed target gene, can be packaged and delivered as AAV |