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Detection of PCNA modifications in Saccharomyces cerevisiae.

Davies AA, Ulrich HD
Methods Mol Biol. 2012;920:543-67. doi: 10.1007/978-1-61779-998-3_36. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
99539YEp181-CUP1-His-UbiYeast episomal vector for expression of His-tagged ubiquitin; LEU2 marker
99540YEp195-CUP1-His-UbiYeast episomal vector for expression of His-tagged ubiquitin; URA3 marker
99541pRS423-CUP1-His-UbiYeast episomal vector for expression of His-tagged ubiquitin; HIS3 marker
99542YIp128-P30-His-POL30Yeast integrative vector for expression of His-tagged POL30 (PCNA); LEU2 marker
99543YIp128-P30-His-pol30(K127R)Yeast integrative vector for expression of His-tagged POL30 (PCNA) mutant K127R; Leu2 marker
99544YIp128-P30-His-pol30(K164R)Yeast integrative vector for expression of His-tagged POL30 (PCNA) mutant K164R; Leu2 marker
99545YIp128-P30-His-pol30(K127/164R)Yeast integrative vector for expression of His-tagged POL30 (PCNA) mutant K127/164R; Leu2 marker
99546YIp211-P30-His-POL30-3'Yeast integrative vector for expression of His-tagged POL30 (PCNA); URA3 marker
99547YIp211-P30-His-pol30(K127R)-3'Yeast integrative vector for expression of His-tagged POL30 (PCNA) mutant K127R; URA3 marker
99548YIp211-P30-His-pol30(K164R)-3'Yeast integrative vector for expression of His-tagged POL30 (PCNA) mutant K164R; URA3 marker
99549YIp211-P30-His-pol30(K127/164R)-3'Yeast integrative vector for expression of His-tagged POL30 (PCNA) mutant K127/164R; URA3 marker
99550YIp204-P30-His-POL30Yeast integrative vector for expression of His-tagged POL30 (PCNA); TRP1 marker

Antibodies from Article