Precision genome editing using CRISPR-Cas9 and linear repair templates in C. elegans.
Paix A, Folkmann A, Seydoux G
Methods. 2017 May 15;121-122:86-93. doi: 10.1016/j.ymeth.2017.03.023. Epub 2017 Apr 7.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 99476 | pAP546-2 | 3XFlag::mNeptune::Myc |
| 99477 | pAP574-1 | 3XFlag::Topaz::Myc |
| 99481 | pAP576-2 | 3XFlag::tagBFP::Myc |
| 99482 | pAP605-1 | Myc::TEV::3XFlag::TEV::Myc |
| 99483 | pAP607-2 | Myc::TEV::3XFlag::GFP::3XFlag::TEV::Myc |
| 99484 | pAP608-1 | H2B::linker::GFP (contain 3 introns)::3XFlag::TEV::Myc::tbb-2 3UTR |
| 99485 | pAP635-2 | Myc::TEV::3XFlag::Dendra2::3XFlag::TEV::Myc |
| 99486 | pAP639-2F | 3XFlag::TEV::6XHis::mTurquoise::6XHis::TEV::3XFlag |
| 99487 | pAP640-2 | 3XFlag::TEV::V5::mOrange2::V5::TEV::3XFlag |
| 99488 | pAP683-2 | TEV::eGFP::ER::HA::linker::3XFlag |
| 99489 | pAP684-3 | TEV::eGFP::ER::Ollas::linker::3XFlag |
| 99490 | pAP685-2 | TEV::eGFP::ER::6xHis::linker::3XFlag |
| 99491 | pAP686-1 | TEV::eGFP::ER::V5::linker::3XFlag |
| 99492 | pAP693-3 | nirFP::3xFlag |
| 99493 | pAP757-1 | 6XHis::TEV::3XFlag::linker::Myc |
| 99494 | pAP758-1 | TEV::eGFP::3XFlag |
| 99495 | pAP835-1 | TEV::meGFP::3XFlag |
| 99496 | pAP973-1 | TEV::linker::meGFP:3XFlag |
| 99497 | pAP974-1 | TEV::linker::tagRFP::3XFlag |
| 99498 | pAP975-1 | TEV::linker::tagBFP::3XFlag |
| 99499 | pAP996-1 | 3XFlag::meGFP::linker::TEV |
| 99500 | pAP997-1 | 3XFlag::tagRFP::linker::TEV |
| 99501 | pAP998-5 | 3XFlag::tagBFP::linker::TEV |
| 99502 | pAP1071-2 | 3XFlag::meGFP with 2 introns::Ollas::H2B::tbb-2 3utr::linker::TEV |