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Photosynthesis-dependent H2O2 transfer from chloroplasts to nuclei provides a high-light signalling mechanism.

Exposito-Rodriguez M, Laissue PP, Yvon-Durocher G, Smirnoff N, Mullineaux PM
Nat Commun. 2017 Jun 29;8(1):49. doi: 10.1038/s41467-017-00074-w. (Link opens in a new window) PubMed

Plasmids from Article

ID Plasmid Purpose
84728pUB-cHyPer2Expresses HyPer2 in plant cells-biosensor for Hydrogen peroxide
84730pUB-nHyPer2 (NLS:HyPer2:NLS)Expresses HyPer2 in plant cells-biosensor for Hydrogen peroxide targeted to Nuclei
84731pUB-nSypHer (NLS:sypHer:NLS)Expresses sypHer in plant cells-biosensor for pH targeted to Nuclei
84732pUB-sSypHer (RbcS-SypHer)Expresses SypHer in plant cells-biosensor for pH targeted to chloroplasts stoma
84733pUB-cSypHerExpresses SypHer in plant cells-biosensor for pH
84734pUB-spHRedExpresses pHRed in plant cells-biosensor for pHRed targeted to chloroplasts stoma
84735pGem-nHyPer:sAPXSimultaneous expresion of stromal HyPer2 and stromal Ascorbate peroxidase (APX) in plant cells
84736pGem-nHyPer:cAPXSimultaneous expresion of stromal HyPer2 and cytosolic Ascorbate peroxidase (APX) in plant cells
84737pGem-nHyPer2:sHyper2Simultaneous expresion of nuclear and stromal HyPer2 in plant cells
84738pGem-sHyper:sAPXSimultaneous expresion of stromal sHyPer and stromal Ascorbate peroxidase (APX) in plant cells

Antibodies from Article