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A calcium- and light-gated switch to induce gene expression in activated neurons.

Lee D, Hyun JH, Jung K, Hannan P, Kwon HB
Nat Biotechnol. 2017 Jun 26. doi: 10.1038/nbt.3902. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
92389pCMV-M13-TEV-C-P2A-TdTomatoexpresses Cal-light M13-TEV-C component and TdTomato in mammalian cells
92390pCMV-TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTAexpresses Cal-light TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA component in mammalian cells
92391pAAV-M13-TEV-C-P2A-TdTomatoproduces AAV expressing Cal-light M13-TevC component and TdTomato
92392pAAV-TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTAproduces AAV expressing Cal-light TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA

Antibodies from Article