Multiplexed Engineering and Analysis of Combinatorial Enhancer Activity in Single Cells.
Xie S, Duan J, Li B, Zhou P, Hon GC
Mol Cell. 2017 Apr 20;66(2):285-299.e5. doi: 10.1016/j.molcel.2017.03.007. Epub 2017 Apr 13.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
89493 | Lenti-sgRNA(MS2)-puro-barcode | Cloning backbone for sgRNA, also has a barcode that allows detection from scRNA-seq |
89567 | Lenti-dCas9-KRAB-blast | Plasmid expression dCas9 protein in fusion with KRAB domain |