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Overexpression of post-translationally modified peptides in Escherichia coli by co-expression with modifying enzymes.

Sugase K, Landes MA, Wright PE, Martinez-Yamout M
Protein Expr Purif. 2008 Feb;57(2):108-15. Epub 2007 Nov 1. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
99343HisGB1-Hif1a(776-826) + CBP TAZ1(340-439)Bacterial expression of mouse CBP TAZ1 domain as coexpression with Hif1a TAD
99508HisGB1-Hif1a(776-826)_FIHBacterial expression of hydroxylated Hif1a TAD (776-826)

Antibodies from Article