Long-term dual-color tracking of genomic loci by modified sgRNAs of the CRISPR/Cas9 system.
Shao S, Zhang W, Hu H, Xue B, Qin J, Sun C, Sun Y, Wei W, Sun Y
Nucleic Acids Res. 2016 May 19;44(9):e86. doi: 10.1093/nar/gkw066. Epub 2016 Feb 4.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
99908 | PB-CMV-NLS-dCas9-NLS | For the expression of NLS-dCas9-NLS |
99909 | pHR-TRE3G-NLS-tdMCP-mCherry | For the expression of NLS-tdMCP-mCherry |
99910 | pHR-TRE3G-NLS-tdPCP-EGFP | For the expression of NLS-tPMCP-EGFP |
99911 | psgRNA2.02-MS2-ccdB | Basic cloning vector for the expression of MS2 inserted sgRNA |
99912 | psgRNA2.02-PP7-ccdB | Basic cloning vector for the expression of PP7 inserted sgRNA |