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Long-term dual-color tracking of genomic loci by modified sgRNAs of the CRISPR/Cas9 system.

Shao S, Zhang W, Hu H, Xue B, Qin J, Sun C, Sun Y, Wei W, Sun Y
Nucleic Acids Res. 2016 May 19;44(9):e86. doi: 10.1093/nar/gkw066. Epub 2016 Feb 4. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
99908PB-CMV-NLS-dCas9-NLSFor the expression of NLS-dCas9-NLS
99909pHR-TRE3G-NLS-tdMCP-mCherryFor the expression of NLS-tdMCP-mCherry
99910pHR-TRE3G-NLS-tdPCP-EGFPFor the expression of NLS-tPMCP-EGFP
99911psgRNA2.02-MS2-ccdBBasic cloning vector for the expression of MS2 inserted sgRNA
99912psgRNA2.02-PP7-ccdBBasic cloning vector for the expression of PP7 inserted sgRNA

Antibodies from Article