A toolkit for GFP-mediated tissue-specific protein degradation in C. elegans.

Wang S, Tang NH, Lara-Gonzalez P, Zhao Z, Cheerambathur DK, Prevo B, Chisholm AD, Desai A, Oegema K
Development. 2017 Jun 15. pii: dev.150094. doi: 10.1242/dev.150094. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
89357	pOD1988-epiDEG	MosSCI targeting vector expressing a fusion between a GFP nanobody and an ubiquitin ligase adaptor ZIF-1 to degrade GFP tagged proteins. Expression is controlled by Pdpy-7 (epidermis specific).
89366	pOD2044-intDEG	MosSCI targeting vector expressing a fusion between a GFP nanobody and an ubiquitin ligase adaptor ZIF-1 to degrade GFP tagged proteins. Expression is controlled by Pelt-2 (intestine specific).
89367	pOD2046-bwmDEG	MosSCI targeting vector expressing a fusion between a GFP nanobody and an ubiquitin ligase adaptor ZIF-1 to degrade GFP tagged proteins. Expression is controlled by Pmyo-3 (body wall muscle specific).
89368	pOD2048-csnDEG	MosSCI targeting vector expressing a fusion between a GFP nanobody and ubiquitin ligase adaptor ZIF-1 to degrade GFP tagged proteins. Expression controlled by Posm-6 (ciliated sensory neuron specific)
89369	pOD2087-trnDEG	MosSCI targeting vector expressing a fusion between a GFP nanobody and an ubiquitin ligase adaptor ZIF-1 to degrade GFP tagged proteins. Expression is controlled by Pmec-18 (touch neuron specific).
100005	pOD2045-intControl	MosSCI targeting vector expressing an ubiquitin ligase adaptor ZIF-1, serving as a control for pOD2044-intDEG. Expression is controlled by Pelt-2 (intestine specific).
100006	pOD2047-bwmControl	MosSCI targeting vector expressing an ubiquitin ligase adaptor ZIF-1, serving as a control for pOD2046-bwmDEG. Expression is controlled by Pmyo-3 (body wall muscle specific).
100007	pOD2049-csnControl	MosSCI targeting vector expressing an ubiquitin ligase adaptor ZIF-1, serving as a control for pOD2048-csnDEG. Expression is controlled by Posm-6 (ciliated sensory neuron specific).