Optimization of CRISPR/Cas9 genome editing for loss-of-function in the early chick embryo.
Gandhi S, Piacentino ML, Vieceli FM, Bronner ME
Dev Biol. 2017 Dec 1;432(1):86-97. doi: 10.1016/j.ydbio.2017.08.036.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 99138 | pCAGG>nls-hCas9-nls | Expresses Cas9 via the chicken beta actin promoter for electroporation in chicken embryos |
| 99139 | U6.3>gRNA.f+e | Empty gRNA scaffold with "Flip" and "Extension" modifications with optimized chicken specific U6 promoter |
| 99140 | U6.3>Control.gRNA.f+e | Control gRNA under the regulation of an optimized chicken specific U6 promoter |
| 99141 | pCAGG>nls-hCas9-nls-GFP | Cas9-GFP fusion protein under the regulation of chicken beta-actin promoter |