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A High-Throughput Mutational Scan of an Intrinsically Disordered Acidic Transcriptional Activation Domain.

Staller MV, Holehouse AS, Swain-Lenz D, Das RK, Pappu RV, Cohen BA
Cell Syst. 2018 Mar 1. pii: S2405-4712(18)30052-8. doi: 10.1016/j.cels.2018.01.015. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
99048pMVS102_P3-GFP-NATMX6EGFP reporter with six binding sites for the Zif268 DNA binding domain
99049pMVS142_pACT1_mCherry_Zif268_EBD_MCS_KANTranscription Factor chassis for activation domains. mCherry, Zif268 DNA binding domain, estrogen response domain

Antibodies from Article