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Elastin-like Polypeptide Linkers for Single-Molecule Force Spectroscopy.

Ott W, Jobst MA, Bauer MS, Durner E, Milles LF, Nash MA, Gaub HE
ACS Nano. 2017 Jun 12. doi: 10.1021/acsnano.7b02694. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
90472pET28a-Cys-ELP(120nm)-LPETGGElastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]6, with N-Terminal Cysteine and C-Terminal Sortase Recognition Sequence LPETGG
90475pET28a-Cys-ELP(40nm)-LPETGGElastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]2, with N-Terminal Cysteine and C-Terminal Sortase Recognition Sequence LPETGG
91571pET28a-GGG-ELP(40nm)-CysElastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]2, with N-Terminal Sortase Recognition Sequence GGG and a C-terminal Cysteine
91572pET28a-GGG-ELP(120nm)-CysElastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]6, with N-Terminal Sortase Recognition Sequence GGG and a C-terminal Cysteine
91697pET28a-CohIII-CBM-HIS-LPETGGCohIII-CBM handle with C-Terminal Sortase Recognition Sequence LPETGG for Posttranslational Coupling to a N-terminal GGG
91698pET28a-GGG-HIS-CBM-Xmod-DocIIICBM-Xmod-DocIII handle with N-Terminal Sortase Recognition Sequence GGG for posttranslational coupling to a C-Terminal LPETGG

Antibodies from Article