Elastin-like Polypeptide Linkers for Single-Molecule Force Spectroscopy.
Ott W, Jobst MA, Bauer MS, Durner E, Milles LF, Nash MA, Gaub HE
ACS Nano. 2017 Jun 12. doi: 10.1021/acsnano.7b02694.
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Article
Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
90472 | pET28a-Cys-ELP(120nm)-LPETGG | Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]6, with N-Terminal Cysteine and C-Terminal Sortase Recognition Sequence LPETGG |
90475 | pET28a-Cys-ELP(40nm)-LPETGG | Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]2, with N-Terminal Cysteine and C-Terminal Sortase Recognition Sequence LPETGG |
91571 | pET28a-GGG-ELP(40nm)-Cys | Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]2, with N-Terminal Sortase Recognition Sequence GGG and a C-terminal Cysteine |
91572 | pET28a-GGG-ELP(120nm)-Cys | Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]6, with N-Terminal Sortase Recognition Sequence GGG and a C-terminal Cysteine |
91697 | pET28a-CohIII-CBM-HIS-LPETGG | CohIII-CBM handle with C-Terminal Sortase Recognition Sequence LPETGG for Posttranslational Coupling to a N-terminal GGG |
91698 | pET28a-GGG-HIS-CBM-Xmod-DocIII | CBM-Xmod-DocIII handle with N-Terminal Sortase Recognition Sequence GGG for posttranslational coupling to a C-Terminal LPETGG |