A Multiplex Genome Editing Method for Escherichia coli Based on CRISPR-Cas12a.
Ao X, Yao Y, Li T, Yang TT, Dong X, Zheng ZT, Chen GQ, Wu Q, Guo Y
Front Microbiol. 2018 Oct 9;9:2307. doi: 10.3389/fmicb.2018.02307. eCollection 2018.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 98592 | p46Cpf1-OP2 | Produces lambda Red and Cpf1 for recombination and selection. The plasmid is combined with a donor plasmid (with crRNA and template) for rapid genome editing in E.coli. |