Adaptation of Tri-molecular fluorescence complementation allows assaying of regulatory Csr RNA-protein interactions in bacteria.
Gelderman G, Sivakumar A, Lipp S, Contreras L
Biotechnol Bioeng. 2015 Feb;112(2):365-75. doi: 10.1002/bit.25351. Epub 2014 Sep 26.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 98584 | pCML 314 | pCsrA-NYFP + CsrB-2MS2BD. Expresses protein-NYFP and RNA-2MS2BD fusions in E. coli for TriFC assay (ampR). |
| 98586 | pCML 313 | pNYFP + CsrB-2MS2BD. Expresses NYFP control fusion and RNA-2MS2BD fusion in E. coli for TriFC assay (ampR). |
| 98587 | pCML 221 | pMS2-CYFP. Expresses MS2 protein-CYFP fusion in E. coli for TriFC assay (kanR). |
| 98588 | pCML 220 | pCYFP. Expresses CYFP control fusion in E. coli for TriFC assay (kanR). |