A general mechanism of photoconversion of green-to-red fluorescent proteins based on blue and infrared light reduces phototoxicity in live-cell single-molecule imaging.
Turkowyd B, Balinovic A, Virant D, Golz Carnero HG, Caldana F, Endesfelder M, Bourgeois D, Endesfelder U
Angew Chem Int Ed Engl. 2017 Jun 2. doi: 10.1002/anie.201702870.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 98571 | pRSETa-mEos3.2-A69T | photoconvertible fluorescent protein mEos3.2-A69T in pRSetA plasmid, photoconvertible by primed conversion mechanism, bacterial overexpression, sequence is codon optimised for E. coli |
| 98572 | pRSETa-mEos4b-V69T | photoconvertible fluorescent protein mEos4b-V69T in pRSetA plasmid, photoconvertible by primed conversion mechanism, bacterial overexpression |
| 98574 | pRSETa-mKikGR-V69T | photoconvertible fluorescent protein mKikGR-V69T in pRSetA plasmid, photoconvertible by primed conversion mechanism, bacterial overexpression, sequence is codon optimised for E. coli |
| 98577 | pRSETa-mMaple3-V157I | photoconvertible fluorescent protein mMaple3-V157I in pRSetA plasmid, photoconvertible by primed conversion mechanism, bacterial overexpression, sequence is codon optimised for E. coli |
| 98578 | pRSETb-pcDronpa2-A69T | photoconvertible fluorescent protein pcDronpa2-A69T in pRSetB plasmid, photoconvertible by primed conversion mechanism, bacterial overexpression |