Coupling gene regulatory patterns to bioprocess conditions to optimize synthetic metabolic modules for improved sesquiterpene production in yeast.
Peng B, Plan MR, Carpenter A, Nielsen LK, Vickers CE
Biotechnol Biofuels. 2017 Feb 21;10:43. doi: 10.1186/s13068-017-0728-x. eCollection 2017.
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PubMed
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 83557 | pILGFPB6 | Used to evaluate the expression output of GAL10 promoter with yEGFP used as the reporter gene. |
| 83558 | pILGFPQ3 | Used to evaluate the expression output of GAL2 promoter with yEGFP used as the reporter gene. |
| 83559 | pILGFPQ4 | Used to evaluate the expression output of GAL7 promoter with yEGFP used as the reporter gene. |
| 98300 | pPMVAugw | Enhancing the upper-part mevalonate pathway; overexpressing codon-optimized Enterococcus faecalis mevalonate pathway genes under the control of CUP1 promoter. |
| 98301 | pIMVAu1 | Enhancing the upper-part mevalonate pathway; overexpressing codon-optimized Enterococcus faecalis mevalonate pathway genes under the control of galactose-inducible promoters; integrated into genome HMG2 locus; overexpressing HMG2 K6R. |
| 98302 | pPMVAd36 | Enhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters or CUP1 promoter. |
| 98303 | pIMVAd39T | Enhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters or GAL2 promoter; integrated into PDC5 locus; disrupting PDC5. |
| 98304 | pJT3 | Overexpressing yeast farnesyl pyrophosphate synthase Erg20p and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of CUP1 promoter |
| 98305 | pJT9R | Overexpressing yeast farnesyl pyrophosphate synthase Erg20p and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of GAL1/GAL2 promoter |