A squalene synthase protein degradation method for improved sesquiterpene production in Saccharomyces cerevisiae.
Peng B, Plan MR, Chrysanthopoulos P, Hodson MP, Nielsen LK, Vickers CE
Metab Eng. 2017 Jan;39:209-219. doi: 10.1016/j.ymben.2016.12.003. Epub 2016 Dec 8.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 83561 | pILGFPJ1 | Express yEGFP-ERG9 ER signal peptide under the control of TEF1 promoter |
| 83562 | pILGFPU0 (R) | Express yEGFP-ERG9 ER targeting peptide-CLN2PEST under the control of TEF1 promoter |
| 98296 | pPMVAu5 (R) | Enhancing the upper-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters. |
| 98297 | pPMVAu8 | Enhancing the upper-part mevalonate pathway; overexpressing codon-optimized Enterococcus faecalis mevalonate pathway genes under the control of consitutive promoters. |
| 98298 | pPMVAd3 | Enhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters. |
| 98299 | pJT1 | Overexpressing yeast farnesyl pyrophosphate synthase Erg20p and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of TEF1/TEF2 promoter |