A Fluorescent Split Aptamer for Visualizing RNA-RNA Assembly In Vivo.

Alam KK, Tawiah KD, Lichte MF, Porciani D, Burke DH
ACS Synth Biol. 2017 May 26. doi: 10.1021/acssynbio.7b00059. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
87307	pUC19-T7-SdB-T	Expresses the fluorescence-activating Stabilized dimeric Broccoli (SdB) aptamer under the control of the T7 promoter.
87308	pUC19-T7-3WJdB-T	Expresses the fluorescence-activating Three-way Junction dimeric Broccoli (3WJdB) aptamer under the control of the T7 promoter.
87309	pUC19-T7-Top-T	Expresses the "Top" transcript of the Split-Broccoli system under the control of the T7 promoter.
87310	pUC19-T7-Bottom-T	Expresses the "Bottom" transcript of the Split-Broccoli system under the control of the T7 promoter.
87311	pUC19-P70a-3WJdB-T	Expresses the fluorescence-activating Three-way Junction dimeric Broccoli (3WJdB) aptamer under the control of the E. coli sigma 70 promoter.
87312	pUC19-P70a-Top-T	Expresses the "Top" transcript of the Split-Broccoli system under the control of the E. coli sigma 70 promoter.
87313	pUC19-P70a-Bottom-T	Expresses the "Bottom" transcript of the Split-Broccoli system under the control of the E. coli sigma 70 promoter.
87314	pUC19-P70a-Top-T~P70a-Bottom-T	Expresses the modular Split-Broccoli system (Top + Bottom) under the control of E. coli sigma 70 promoters.
87315	pUC19-P70a-Top-T~Bottom-T	Control plasmid. Encodes the Split-Broccoli system (Top + Bottom) with only "Top" under the control of E. coli sigma 70 promoter.
87316	pUC19-Split-Broccoli-Toehold-Switch	Expresses the Split-Broccoli system and a Trigger/Toehold switch for mCherry.
87317	pUC19-Trigger-Bottom	Expresses the "Bottom" transcript of the Split-Broccoli system downstream of an RNA Toehold switch Trigger sequence.
87318	pUC19-Top-Toehold-mCherry	Expresses the "Top" transcript of the Split-Broccoli system upstream of an RNA Toehold switch controlling mCherry.