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Fluorescence lifetime imaging microscopy reveals rerouting of SNARE trafficking driving dendritic cell activation.

Verboogen DRJ, Gonzalez Mancha N, Ter Beest M, van den Bogaart G
Elife. 2017 May 19;6. pii: e23525. doi: 10.7554/eLife.23525. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
92420pmCitrine_N1Empty backbone for C-terminal fusion of mCitrine
92421Stx3-pmCitrineExpression of syntaxin-3 C-terminally conjugated to mCitrine.
92422Stx4-pmCitrineExpression of syntaxin-4 C-terminally conjugated to mCitrine.
92423VAMP3-mCherryExpression of VAMP3 C-terminally conjugated to mCherry.
92424VAMP8-mCherryExpression of VAMP8 C-terminally conjugated to mCherry.
92425VAMP3(Δ71)-mCherryExpression of fusion incompetent VAMP3 (del71) C-terminally conjugated to mCherry.
92426Stx3-mCitrine-mCherryExpression of syntaxin-3 C-terminally conjugated to tandem of mCitrine and mCherry.
92427FKBP-Stx4-mCitrineExpression of syntaxin-4 N-terminally conjugated to FKBP and C-terminally conjugated to mCitrine.
92428FKBP-Stx3-mCitrineExpression of syntaxin-3 N-terminally conjugated to FKBP and C-terminally conjugated to mCitrine.
92429FRB_T2098L-VAMP3-mCherryExpression of VAMP3 N-terminally conjugated to FRB and C-terminally conjugated to mCitrine.

Antibodies from Article