Characterization of the Fast and Promiscuous Macrocyclase from Plant PCY1 Enables the Use of Simple Substrates.
Ludewig H, Czekster CM, Oueis E, Munday ES, Arshad M, Synowsky SA, Bent AF, Naismith JH
ACS Chem Biol. 2018 Mar 16;13(3):801-811. doi: 10.1021/acschembio.8b00050. Epub 2018 Feb 12.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 98150 | pJ414-PCY1 | Expresses PCY1 from Saponaria vaccaria possessing a N-terminal His6-tag (TEV cleavable) in E. coli. |
| 98151 | pJ414-PCY1:S495A | Expresses PCY1:S495A from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli. |
| 98153 | pJ414-PCY1:W603A | Expresses PCY1:W603A from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli. |
| 98154 | pJ414-PCY1:H695Q | Expresses PCY1:H695Q from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli. |
| 98155 | pJ414-PCY1:H695A | Expresses PCY1H695A from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli. |
| 98156 | pJ414-PCY1:Y696G | Expresses PCY1:R696G from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli. |