Nontransgenic Marker-Free Gene Disruption by an Episomal CRISPR System in the Oleaginous Microalga, Nannochloropsis oceanica CCMP1779.
Poliner E, Takeuchi T, Du ZY, Benning C, Farre EM
ACS Synth Biol. 2018 Apr 20;7(4):962-968. doi: 10.1021/acssynbio.7b00362. Epub 2018 Mar 14.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 98147 | pNOC-CRISPR-P2A-BlastR | Expresses Cas9-Nlux-P2A-BlastR and sgRNA in Nannochloropsis oceanica. |
| 99369 | pNOC-CRISPR-compact | Expresses Cas9-Nlux and sgRNA. Contains CEN/ARS. No selection marker. |
| 99370 | pNOC-CRISPR-HA | Expresses Cas9-HA and sgRNA. Hygromycin resistance. |
| 99861 | pNOC-CRISPR-35S | Expresses Cas9-Nlux and sgRNA. Contains CEN/ARS and 35 terminator. No selection marker. |
| 99863 | pNOC-ARS-CRISPR-v2 | Expresses Cas9-Nlux and sgRNA. Compact backbone contains CEN/ARS. Hygromycin selection marker. |
| 100008 | pNOC-CRISPR | Expresses Cas9-Nlux and sgRNA in Nannochloropsis oceanica, contains hygromycin resistance cassette, for integration into genome |
| 100009 | pNOC-CRISPR-GFP | Expresses Cas9-GFP and sgRNA in Nannochloropsis oceanica, contains hygromycin resistance cassette, for integration into geneome |
| 101009 | pNSA-ARS-CRISPR-BlastR | Expresses Cas9-Nlux and sgRNA and contains CEN/ARS. CCMP537 LDSP promoter driven Blasticidin resistance gene. |