Temporally precise labeling and control of neuromodulatory circuits in the mammalian brain.
Lee D, Creed M, Jung K, Stefanelli T, Wendler DJ, Oh WC, Mignocchi NL, Luscher C, Kwon HB
Nat Methods. 2017 May;14(5):495-503. doi: 10.1038/nmeth.4234. Epub 2017 Apr 3.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 89869 | pCMV-bArrestin2-TevC-P2A-TdTomato | expresses iTango bArrestin2-TevC component and TdTomato bicistronically |
| 89870 | pCMV-DRD2-V2tail-TevN-BLITz1-TetR-VP16 | expresses DRD2-iTango component in mammalian cells |
| 89871 | pTRE-EGFP | EGFP reporter vector for iTango system |
| 89872 | pCMV-CB1R-V2tail-TevN-BLITz1-TetR-VP16 | expresses CB1R-iTango component in mammalian cells |
| 89873 | pAAV-hSYN-bArrestin2-TevC-P2A-TdTomato-WPRE-bGHpA | expresses iTango bArrestin2-TevC component and TdTomato bicistronically for AAV production |
| 89874 | pAAV-hSYN-DRD2-V2tail-TevN-BLITz1-TetR-VP16-bGHpA | expresses DRD2-iTango component in mammalian cells for AAV production |
| 89875 | pAAV-TRE-EGFP | EGFP reporter vector of iTango system for AAV production |
| 89876 | pAAV-TRE-FLEX-eNpHR-EYFP | To generate AAV virus that will express eNpHR-EYFP in Cre-dependent recombination under control of tetO promoter |
| 89877 | pCMV-NES-CRY2PHR-TevC | expresses NES-CRY2PHR-TevC of BLITz system |
| 89878 | pCMV-TM-CIBN-BLITz1-TetR-VP16 | expresses TM-CIBN-BLITz1-TetR-VP16 of BLITz system |