Live cell imaging of low- and non-repetitive chromosome loci using CRISPR-Cas9.
Qin P, Parlak M, Kuscu C, Bandaria J, Mir M, Szlachta K, Singh R, Darzacq X, Yildiz A, Adli M
Nat Commun. 2017 Mar 14;8:14725. doi: 10.1038/ncomms14725.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 101152 | sg2.02 MUC4.1 | gRNA with two MCP binding sites |
| 101153 | sg14x(MS2) MUC4.1 | gRNA with 14 MCP binding site |
| 101154 | sg2.02 16x(MS2) MUC4.1 | gRNA with 16 MCP binding site |
| 101155 | sg2.0 (PP7) Locus#2 | gRNA with two PCP binding sites |
| 101160 | MCP-YFP | to express MCP-YFP fusion protein |
| 101161 | PCP-GFP | to epress PCP-GFP fusion protein |