An efficient method to enrich for knock-out and knock-in cellular clones using the CRISPR/Cas9 system.
Niccheri F, Pecori R, Conticello SG
Cell Mol Life Sci. 2017 Apr 18. doi: 10.1007/s00018-017-2524-y.
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PubMed
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 54321 | pBS SK mCherryROSAbsr | Encodes mCherry fused with out-of-frame blasticidin-S resistance gene with a linker containing a target for TALEN/CRISPR. Can use as a surrogate target to select cells expressing active TALEN/CRISPR. |
| 54322 | pBS SK mCherryROSAegfp | Encodes mCherry fused with an out-of-frame EGFP through a linker containing a target for TALEN/CRISPR. Can be used as a surrogate target to test the specificity of TALENs and CRISPR. |
| 66950 | pBSR (mCherry-surrogateTarget-bsr) | Encodes mCherry fused with out-of-frame blasticidin-S resistance gene with a linker containing a target for TALEN/CRISPR. Can use as a surrogate target to select cells expressing active TALEN/CRISPR. |