LEM2 recruits CHMP7 for ESCRT-mediated nuclear envelope closure in fission yeast and human cells.
Gu M, LaJoie D, Chen OS, von Appen A, Ladinsky MS, Redd MJ, Nikolova L, Bjorkman PJ, Sundquist WI, Ullman KS, Frost A
Proc Natl Acad Sci U S A. 2017 Mar 14;114(11):E2166-E2175. doi: 10.1073/pnas.1613916114. Epub 2017 Feb 27.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 96971 | pMGF130 | S. pombe lem2 knockout cassette with hygro resistance gene |
| 96972 | pMGF157 | ish1-mCherry insertion cassette with Hygro resistance gene |
| 96996 | pMGF169 | ish1 mCherry insertion cassette with G418 resistance gene |
| 96997 | pMGF170 | ish1-mCherry insertion cassette with NAT resistance gene |
| 96998 | pMGF172 | ish1 mCherry insertion cassette with ura4+ gene |
| 96999 | pMGF173 | NLS-GFP-lacZ leu1 insertion vector |
| 97005 | pMGF196 | Expresses LEMD2-mCherry in mammalian cells under a crippled promoter |
| 97006 | pMGF182 | Expresses GFP-CHMP7 in mammalian cells under a crippled promoter |