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Metabolomics-assisted proteomics identifies succinylation and SIRT5 as important regulators of cardiac function.

Sadhukhan S, Liu X, Ryu D, Nelson OD, Stupinski JA, Li Z, Chen W, Zhang S, Weiss RS, Locasale JW, Auwerx J, Lin H
Proc Natl Acad Sci U S A. 2016 Apr 19;113(16):4320-5. doi: 10.1073/pnas.1519858113. Epub 2016 Apr 5. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
96887pET-Duet-ECHA(37-763)-FLAG (no His-tag)expresses flag-tagged mouse ECHA (37-768) in E.coli cells (no His-tag)
96888pET-Duet-ECHA(37-763) K351R-FLAG (no His-tag)expresses-flag tagged mouse ECHA(37-768) K351R in E.coli cells (no His-tag)
96890pet-ECHB(35-475)expresses His-tagged mouse ECHB (35-475) in E.coli cells

Antibodies from Article